293F Hi-Exp Medium

293F Hi-Exp Medium, used for Fc-fusion secreted protein expression, outperformed a competitor's system in protein production, showing a >2-fold titer increase (190 mg/L vs. 90 mg/L), with VCD and viability monitored during expression and protein integrity.

293F Hi-Exp Medium Product Insert - coming soon
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Guides and Flyers

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Which 293 cell lines are compatible with this expression system?

This system is validated for a wide range of suspension 293 cell lines, including HEK293F and HEK293S.

Is this expression system entirely serum-free?

Yes. All components of the system are 100% serum-free.

What is the optimal timing for protein harvest?

For secreted proteins, we recommend harvesting when cell viability drops to ≤60%.

For intracellular or membrane proteins, harvest at a viability threshold of ≤85%, or adjust based on the specific protein stability.

Is the 293F Hi-Exp Medium compatible with Strep-tag or His-tag purification systems?

For intracellular or membrane proteins, the medium is fully compatible with Strep-tag or His-tag purification systems.

For secreted proteins, the medium can be compatible with His-tag purification systems, but it is not directly compatible with Strep-tag purification systems. It is recommended to perform a buffer exchange or dialyze the samples before Strep-tag purification.

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